Recombinant Human TSG101, His-tagged

• Cat.No.: TSG101-30749TH
• Product Overview: Recombinant fragment of Human TSG101 fused to a 36 amino acid His-Tag at N-terminus, 20.7kDa.

Specification

• Description: The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.
• Protein length: 145 amino acids
• Conjugation: HIS
• Molecular Weight: 20.700kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: pH: 8.00Constituents:0.32% Tris HCl, 0.02% DTT, 10% Glycerol
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAVS ESQLKKMVSK YKYRDLTVRE TVNVITLYKD LKPVLDSYVF NDGSSRELMN LTGTIPVPYR GNTYNIPICL WLLDTYPYNP PICFVKPTSS MTIKTGKHVD ANGKIYLPYL HEWKHPQSDL LGLIQVMIVV FGDEPPVFSR P.
• Sequence Similarities: Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.Contains 1 SB (steadiness box) domain.Contains 1 UEV (ubiquitin E2 variant) domain.

Gene Information

• Gene Name: TSG101 tumor susceptibility gene 101 [ Homo sapiens ]
• Official Symbol: TSG101
• Synonyms: TSG101; tumor susceptibility gene 101; TSG10, tumor susceptibility gene 10; tumor susceptibility gene 101 protein; VPS23
• Gene ID: 7251
• mRNA Refseq: NM_006292
• Protein Refseq: NP_006283
• MIM: 601387
• Uniprot ID: Q99816
• Chromosome Location: 11p15
• Pathway: ESCRT-I complex, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; Endosomal Sorting Complex Required For Transport (ESCRT), organism-specific biosystem; Glucocorticoid receptor regulatory network, organism-specific biosystem
• Function: DNA binding; calcium-dependent protein binding; ligand-dependent nuclear receptor transcription coactivator activity; protein binding; transcription corepressor activity