| Cat. No. : |
GSTA1-489H |
| Description : |
GSTA1, also known as Glutathione S-transferase alpha 1, belongs to a glutathione S-transferase alpha class and is the most abundantly expressed in liver. This protein functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. |
| Sequences of amino acids : |
MAEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF |
| Form : |
Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT 10% glycerol |
| Molecular Weight : |
25.6 kDa (222aa) |
| Purity : |
> 90% by SDS - PAGE |
| Concentration : |
1 mg/ml (determined by Bradford assay) |
| Biological activity : |
Specific activity is 5-8 units/mg, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25°C. |
| Activity Assay : |
1.Prepare a 1.mll reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA, 2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB), 3.2% (v/v) ethanol.2.Equilibrate to 25°C and monitor the A340nm until the value is constant using a spectrophotometer. 3.Add 50ul of recombinant GSTA1 protein with various concentrations (1ug, 2ug, 5ug) in 950ul reaction buffer. 4.Mix by inversion and record the increase in A340nm for 5 minutes. |
| Storagee : |
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
| GeneIDD : |
2938 |
| Pathways : |
Drug metabolism - cytochrome P450; Glutathione metabolism; Metabolism of xenobiotics by cytochrome P450; Biological oxidations. |
