| Cat. No. : |
EPGN-41H |
| Description : |
Epigen is an EGF-related polypeptide growth factor that signals through the ErbB receptor-1. It is produced in several tissues, including the testis, liver, heart and in certain tumor cells. Epigen is mitogenic for fibroblasts and epithelial cells. Human Epigen is initially synthesized as a glycosylated 14.7 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a mature soluble sequence. |
| Source : |
Escherichia Coli. |
| Molecular Weight : |
Approximately 7.9kDa monomeric protein, containing 72 amino acid residues, which comprises the EGF homologous portion of the Epigen precursor. |
| AA Sequence : |
AVTVTPPITA QQADNIEGPI ALKFSHLCLE DHNSYCINGA CAFHHEL EKA ICRCFTGYTG ERCEHLTLTS YA. |
| Physical Appearance : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Purity : |
>98% by SDS-PAGE and HPLC analyses. |
| Formulation : |
Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH 7.4. |
| Endotoxin : |
Less than 1EU/μg of rHuEPG as determined by LAL method. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions. |
| Storage : |
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles. |
