||Recombinant Human Apoliprotein-D His Tag fusion protein at C-terminus produced inE.Coliis a single, non-glycosylated, Polypeptide chain containing 174 amino acids and having a molecular mass of 19.8 kDa. The Apoliprotein-D is purified by proprietary chromatographic techniques.
||Apolipoprotein-D is mainly associated with high density lipoproteins in human plasma. Apolipoprotein-D is an atypical apolipoprotein and, based on its primary structure, Apolipoprotein-D is a member of the lipocalin family. Lipocalins adopt a beta-barrel tertiary structure and transport small hydrophobic ligands. Apolipoprotein-D binds cholesterol, progesterone, pregnenolone, bilirubin and arachidonic acid. Apolipoprotein-D is expressed in numerous tissues having high levels of expression in spleen, testes and brain. Apolipoprotein-D is present at high concentrations in the cyst fluid of women with gross cystic disease of the breast, a condition associated with increased risk of breast cancer. Apolipoprotein-D is a multi-ligand, multi-functional transporter and transports a ligand from 1 cell to another within an organ, scavenge a ligand within an organ for transport to the blood or could transport a ligand from the circulation to specific cells within a tissue. The protein a.a.sequence corresponds to the UniProtKB/Swiss-Prot entry P05090. The Following gene modifications were made: Trp99His, Cys116Ser, Ile118Ser, Leu120Ser amino acids exchanges were introduced at the surface of Apoliprotein-D to enhance the protein"s solubility and another three Leu23Pro, Pro133Val, Asn134Ala amino acids exchnages which facilitate its genetic manipulation.
||Greater than 95% as determined by SDS-PAGE.
||Add deionized H2O to a working volume of 0.5mg/ml and let the lyophilized pellet dissolve completely.
||Sterile Filtered White lyophilized (freeze-dried) powder.
||Lyophilized from 1mg/ml in 4mM KH2PO4, 16mM Na2HPO4 and 115mM NaCl pH7.5.
||Store lyophilized protein at -20℃. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4℃. for a limited period of time; it does not show any change after two weeks at 4℃. The lyophilized protein remains stable until the expiry date when stored at -20℃.