||Recombinant Human dickkopf homolog 1 (Xenopus laevis) is expressed inE. coli.DKK1 is a 35-40 kDa glycoprotein that contains 235 amino-acid residues.
||Recombinant Human DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play an important regulatory role in the Wnt /β-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/βcatenin signaling system. LPR5 and LPR6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/βcatenin signaling cascade. It has been suggested that by inhibiting Wnt/β-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis.
||Greater than 97% by SDS-PAGE gel and HPLC analyses.
||Endotoxin level is less than 0.1 ng per μg (1EU/μg).
||TLNSVLNSNAIKNL PPPLGGAAGHPGSAVS AAPGILYPGGNKYQ TIDNYQPYPCAE DEECGTD EYCASPTRGG DAGVQICLACR KRRKRCMR HAMCCPGN YCKNGI CVSS DQNHFRGEIE ETITE SFGNDHSTLDGYSRR TTLSSKMYHT KGQEGS VCLR SSDCASGLCCARHFWSKICK PVLKEGQVCT KHRRKGSHGL EIFQRCYCGE GLS CRIQK DH HQASNSSRLH TCQRH.
||The lyophilized protein is stable for at least 2 years from date of receipt at -20℃. Reconstituted DKK-1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20℃. Avoid repeated freeze/thaw cycles.