"ANGPTL3" Related Products

Expand
Expand
Expand

Recombinant Human ANGPTL3, Fibrinogen-like Domain, FLAG-tagged

Cat. No.: ANGPTL3-399H
Product Overview: Recombinant Human Angiopoietin-like 3 is expressed inHEK 293 cells.The Angiopoietin-like protein with FLAG-tagged is 26kDa and containing 207 amino acid.
Description: Angiopoietin-like proteins ANGPTL3 and ANGPTL4 are secreted proteins mainly expressed in liver that have been demonstrated to regulate triglyceride metabolism by inhibiting the lipolysis of triglyceride-rich lipoproteins. ANGPTL3 is structurally similar to angiopoietins, which are vascular endothelial growth factors. The experimental results show that Angptl3 and Angptl4 function to regulate circulating triglyceride levels during different nutritional states and therefore play a role in lipid metabolism during feeding/fasting through differential inhibition of Lipoprotein lipase (LPL). Using deletion mutants of Angiopoietin 5 human, it was demonstrated that the N-terminal domain containing fragment (17–207) and not the C-terminal fibrinogen-like domain containing fragment (207–460) increased the plasma triglyceride levels in mice.
Source: HEK 293.
Purity: Greater than 95% as determined by SDS-PAGE.
Formulation: Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.
Amino Acid Sequence: MRGSHHHHHH GMASHMSRID QDNSSFDSLS PEPKSRFAML DDVKILANGL LQLGHGLKDF VHKTKGQIND IFQKLNIFDQ SFYDLSLQTS EIKEEEKELR RTTYKLQVKN EEVKNMSLEL NSKLESLLEE KILLQQKVKY LEEQLTNLIQ NQPETPEHPE VTSLKTFVEK QDNSIKDLLQ TVEDQYKQLN QQHSQIKEIE NQLRRTSIQE PTEISLSSKP RAP.
Biological Activity: The biological activity was determined by the induction of endothelial cell sprouting as described in Korff et al., 2001.
Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of ANG-2 as a Reference Standard.
Specificity: The amino acid sequence of the recombinant Human ANGPTL3 is 100% homologous to the 17-223 amino acid sequence of the Human Angiopoietin-like proteins-3 precursor without signal sequence.
Storage: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°Cfor a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C.
Gene Name: ANGPTL3 angiopoietin-like 3 [ Homo sapiens ]
Synonyms: ANGPTL3; angiopoietin-like 3; ANGPT5; angiopoietin 5; angiopoietin-related protein 3; Angiopoietin-5; ANG-5; UNQ153/PRO179; OTTHUMP00000010719
Gene ID: 27329
mRNA Refseq: NM_014495
Protein Refseq: NP_055310
MIM: 604774
UniProt ID: Q9Y5C1
Chromosome Location: 1p31.1-p22.3
Function: cell surface binding; enzyme inhibitor activity; growth factor activity; integrin binding; phospholipase inhibitor activity

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.