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| Cat. No.: | CASP4-599H |
| Product Overview: | Recombinant Human CASP4 was produced inE. coli. |
| Description: | Caspase-4 is a member of the caspase family of cysteine proteases. It exists in cells as an inactive pro-enzyme. The pro-enzyme is matured by proteolysis to yield large and small subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. The expressed caspase-4 spontaneously undergoes auto-processing to yield subunits characteristic of the native enzyme. The active caspase-4 is routinely tested for its ability to enzymatically cleave the substrate WEHD-AFC or WEHD-pNA. |
| Source: | E. coli. |
| Purity: | ≥95% (SDS-PAGE). |
| Formulation: | Lyophilized. |
| Reconstitution: | Reconstitute with PBS containing 15% glycerol to 1U/µl. |
| Specific Activity: | ~5"000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate WEHD-pNA per hour at 37°C in reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT. |
| Application: | Useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity. |
| Storage: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80℃. |
| Gene Name: | CASP4 caspase 4, apoptosis-related cysteine peptidase [ Homo sapiens ] |
| Synonyms: | CASP4; caspase 4, apoptosis-related cysteine peptidase; TX; ICH-2; Mih1/TX; ICEREL-II; ICE(rel)II; caspase-4; CASP-4; ICE(rel)-II; protease TX; protease ICH-2; apoptotic cysteine protease Mih1/TX; caspase 4, apoptosis-related cysteine protease; EC 3.4.22.57;TX protease; Caspase-4 subunit 1; Caspase-4 subunit 2; ICH2 |
| Gene ID: | 837 |
| mRNA Refseq: | NM_001225 |
| Protein Refseq: | NP_001216 |
| MIM: | 602664 |
| UniProt ID: | P49662 |
| Chromosome Location: | 11q22.2-q22.3 |
| Function: | cysteine-type endopeptidase activity; peptidase activity; protein binding |
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