"CASP4" Related Products

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Recombinant Human Caspase 4, Apoptosis-related Cysteine Peptidase

Cat. No.: CASP4-599H
Product Overview: Recombinant Human CASP4 was produced inE. coli.
Description: Caspase-4 is a member of the caspase family of cysteine proteases. It exists in cells as an inactive pro-enzyme. The pro-enzyme is matured by proteolysis to yield large and small subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. The expressed caspase-4 spontaneously undergoes auto-processing to yield subunits characteristic of the native enzyme. The active caspase-4 is routinely tested for its ability to enzymatically cleave the substrate WEHD-AFC or WEHD-pNA.
Source: E. coli.
Purity: ≥95% (SDS-PAGE).
Formulation: Lyophilized.
Reconstitution: Reconstitute with PBS containing 15% glycerol to 1U/µl.
Specific Activity: ~5"000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate WEHD-pNA per hour at 37°C in reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
Application: Useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
Storage: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80℃.
Gene Name: CASP4 caspase 4, apoptosis-related cysteine peptidase [ Homo sapiens ]
Synonyms: CASP4; caspase 4, apoptosis-related cysteine peptidase; TX; ICH-2; Mih1/TX; ICEREL-II; ICE(rel)II; caspase-4; CASP-4; ICE(rel)-II; protease TX; protease ICH-2; apoptotic cysteine protease Mih1/TX; caspase 4, apoptosis-related cysteine protease; EC 3.4.22.57;TX protease; Caspase-4 subunit 1; Caspase-4 subunit 2; ICH2
Gene ID: 837
mRNA Refseq: NM_001225
Protein Refseq: NP_001216
MIM: 602664
UniProt ID: P49662
Chromosome Location: 11q22.2-q22.3
Function: cysteine-type endopeptidase activity; peptidase activity; protein binding

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