||Recombinant CDK2AP1 protein fused to his-tag was expressed inE.coliand purified by using conventional chromatography techniques. 16.6kDa (152aa).
||CDK2AP1 is a specific CDK2-associated protein, which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. Also, interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggested the regulatory role in DNA replication during S phase of the cell cycle. A similar gene in hamster was isolated from, and functions as a growth suppressor of normal keratinocytes.
||> 95% by SDS – PAGE.
||20mM Tris-HCl buffer (pH8.0) containing 50% glycerol, 2mM DTT.
||0.25 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMSY KPNLAA H MPA AALNAAGSVH SPSTSMATSS QYRQLLSDYG PPSLGYT QGTGNS QV P Q S KY AELLAIIEEL GKEIRPTYAG SKSAMERLKR GIIHARGLVR ECLAET ERNA R S.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.