||Recombinant human SIRT2 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 41.7kDa (372aa).
||SIRT2 (NAD-dependent deacetylase sirtuin-2) is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. SIRT2 is a NAD-dependent deacetylase, which deacetylates the "Lys-40" of alpha-tubulin.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 30% glycerol, 2mM DTT, 200mM NaCl, 0.5mM EDTA.
||> 90% by SDS - PAGE.
||0.25mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MDFLRNLFSQ TLSLGSQKER LLDELTLEGV ARY MQSERCR RVICLVGAGI STSAGIPDFR SPSTGLYDNL EKYHLPYPEA IFEISYFKKH PEPFFALAKE LYPGQFKPTI CHYFMRLLKD KGLLLRCYTQ NIDTLERIAG LEQE DLVEAH GTFYTSHCVSASCRHEYPLS WMKEKIFSEV TPKCEDCQSL VKPDI VF FGE SLPARFFSCM QSDFLKVDLL LVMGTSLQVQ PFASLISKAP LSTPRLLINK EK A GQ SDPFL GMIMGLGGGM DFDSKKAYRD VAWLGECDQG CLALAELLGW KKE LEDL VRR EHASIDAQSG AGVPNPSTSA SPKKSPPPAK DEARTTEREK PQ.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.