||Recombinant human HDHD2 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 30.6 kDa (279aa).
||Haloacid dehalogenase-like hydrolase domain containing 2, also known HDHD2, belongs to the HAD-like hydrolase superfamily. This family of hydrolase enzymes includes L-2-haloacid dehalogenase, epoxide hydrolases and phosphatases. HDHD2 has two active sites, an L-2-haloacid dehalogenase and a carboxylate group. The L-2-haloacid dehalogenase active site catalyzes the hydrolytic dehalogenation of D- and L-2-haloalkanoic acids, producing L- and D-2-hydroxyalkanoic acids.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl.
||> 95% by SDS - PAGE.
||1mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MAACRALKAV LVDLSGTLHI EDAAVPGAQE ALK RLRGASV IIRFVTNTTK ESKQDLLERL RKLEFDISEDEIFTSLTAAR SLLERKQVRP MLLV DDRALP DFKGIQTSDP NAVVMGLAPE HFHYQILNQA FRLLLDGAPL IAIH KARYYK RKDGLALGPG PFVTALEYAT DTKATVVGKP EKTFFLEALR GTG CEPE EAV MIGDDCRDDV GGAQDVGMLG ILVKTGKYRA SDEEKINPPP YLTC ESFP HAVD HILQHLL.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.