||Recombinant human PYCR1 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography.MW = 35.5 kDa(339aa).
||PYCR1 is a universal housekeeping enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. It forms a homopolymer and localizes to the mitochondrion. Defects in PYCR1 are the cause of cutis laxa autosomal recessive type 2B (ARCL2B).
||Liquid. In 20 mM Tris-HCl Buffer (pH 8.5) containing 10% Glycerol.
||> 90% by SDS - PAGE.
||0.5 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MSVGFIGAGQ LAFALAKGFT AAGVLAAHKI MAS SPDMDLA TVSALRKMGV KLTPHNKETV QHSDVLFLAVKPHIIPFILD EIGADIEDRH IVVSC AAGVT ISSIEKKLSA FRPAPRVIRC MTNTPVVVRE GATVYATGTH AQV EDGRLME QLLSSVGFCTEVEEDLIDAV TGLSGSGPAY AFTALDALAD GGVK MGLPRR LAVRLGAQAL LGAAKMLLHS EQHPGQLKDN VSSPGGATIH ALHVL ESG GFRSLLINAVEA SCIRTRELQS MADQEQVSPA AIKKTILDKV KLDSPAGTAL SPS GHTKLLP RSLAPAGKD.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Arginine and proline metabolism; Metabolic pathways; Metabolism of amino acids and derivatives