Cat. No. :	HUS1-3364H
Product Overview :	Recombinant human HUS1 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography.MW = 33.8 kDa (300aa).
Description :	HUS1 is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex. This protein associates with Rad9 and Rad1 to form the 9-1-1(RAD9-RAD1-HUS1) complex, which localizes to DNA lesions and promotes DNA damage signaling and repair or apoptosis, cell cycle arrest. The trimeric complex is structurally similar to the proliferating cell nuclear antigen (PCNA) sliding clamp and interacts with Rad17 as a clamp-clamp loader pair during the DNA damage response.
Source :	E.coli.
Form :	Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 100 mM NaCl, 40% Glycerol.
Purity :	> 95% by SDS - PAGE.
Concentration :	0.25mg/ml (determined by Bradford assay).
Sequences Of Amino Acids :	MGSSHHHHHH SSGLVPRGSH MKFRAKIVDG ACLNHFTRIS NMIAKLAKTC TLRIS PDKLN FILCDKLANG GVSMWCELEQ ENFFNEFQMEGVSAENNEIY LELTSENLSR ALKTA QNARA LKIKLTNKHF PCLTVSVELL SMSSSSRIVT HDIPIKVIPR KLWK DLQEPV VPDPDVSIYLPVLKTMKSVV EKMKNISNHL VIEANLDGEL NLKIETELVC VTTHF KDLGN PPLASESTHE DRNVEHMAEV HIDIRKLLQF LAGQQV NPTKAL CNIVNNKM VHFDLLHEDV SLQYFIPALS.
Storage :	Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Pathways :	Cell Cycle Checkpoints

Gene Name :	HUS1 HUS1 checkpoint homolog (S. pombe) [ Homo sapiens ]
Synonyms :	HUS1 checkpoint homolog (S. pombe); HUS1; checkpoint protein HUS1; hHUS1;hus1+-like protein; OTTHUMP00000208365 ; OTTHUMP00000208366; OTTHUMP00000208378; HUS1 (S. pombe) checkpoint homolog
Gene ID :	3364
mRNA Refseq :	NM_004507
Protein Refseq :	NP_004498
MIM :	603760
UniProt ID :	O60921
Chromosome Location :	7p13-p12
Function :	protein binding; protein serine/threonine kinase activity

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