| Source : |
HEK293 |
| Species : |
Mouse |
| Tag : |
His |
| Form : |
Lyophilized from sterile 25mM Tris, 150mM NaCl, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
| Bio-activity : |
Measured by its ability to hydrolyze pnitrophenylacetate. The specific activity is >50,000 pmoles/min/μg. |
| Molecular Mass : |
The secreted recombinant mouse CES5 comprises 541 amino acids and has a calculated molecular mass of 60.6 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 52 kDa band in SDS-PAGE under reducing conditions. |
| Proteinlength : |
Met1-His556 |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
| Purity : |
> 88 % as determined by SDS-PAGE |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
