Recombinant Human Uroporphyrinogen Decarboxylase, His-tagged

• Cat.No.: UROD-730H
• Product Overview: Recombinant humanUROD protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 43.0 kDa (387aa).

Specification

• Cat. No.: UROD-730H
• Description: UROD is the fifth enzyme in the human heme biosynthetic pathway and is responsible for the conversion of uroporphyrinogen to coproporphyrinogen through the removal of four carboxymethyl side chains. Mutations and deficiency in this enzyme are responsible for three autosomal disorders in humans: familial porphyria cutanea tarda (f-PCT), sporadic porphyria cutanea tarda (s-PCT) and hepatoerythropoietic porphyria (HEP).
• Source: E.coli.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MEANGLGPQG FPELKNDTFL RAAWGEETDY TPVWCMRQAG RYLPEFRETR AAQDFFSTCR SPEACCELTL QPLRRFPLDA AIIFSDILVV PQALGMEVTM VPGKGPSFPE PLREEQDLER LRDPEVVASE LGYVFQAITL TRQRLAGRVP LIGFAGAPWT LMTYMVEGGG SSTMAQAKRW LYQRPQASHQ LLRILTDALV PYLVGQVVAG AQALQLFESH AGHLGPQLFN KFALPYIRDV AKQVKARLRE AGLAPVPMII FAKDGHFALE ELAQAGYEVV GLDWTVAPKK ARECVGKTVT LQVNLDPCAL YASEEEIGQL VKQMLDDFGP HRYIANLGHG LYPDMDPEHV GAFVDAVHKH SRLLRQN
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 20% glycerol 1mM DTT, 0.1M NaCl, and 1mM EDTA.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: UROD uroporphyrinogen decarboxylase [ Homo sapiens ]
• Synonyms: UROD; uroporphyrinogen decarboxylase; PCT; uroporphyrinogen III decarboxylase; fifth enzyme of heme biosynthetic pathway; fifth enzyme of the heme biosynthetic pathway; EC 4.1.1.37; UPD; URO-D; OTTHUMP00000010502
• Gene ID: 7389
• mRNA Refseq: NM_000374
• Protein Refseq: NP_000365
• MIM: 176100
• UniProt ID: P06132
• Chromosome Location: 1p34
• Pathway: Porphyrin and chlorophyll metabolism; Metabolism of porphyrins
• Function: uroporphyrinogen decarboxylase activity; lyase activity