Recombinant Human PRKCI, GST tagged

• Cat.No.: PRKCI-757H
• Product Overview: Recombinant Human PRKCI (NP_002731.4) (Met 10-Val 596), fused with the GST tag at the C-terminus, was produced in Baculovirus-Insect cells.

Specification

• Source: Baculovirus-Insect Cells
• Species: Human
• Tag: GST
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM GSH, 0.5mM PMSF, 10% glycerol, pH 7.4
• Molecular Mass: The recombinant human PKCλ/GST chimera consists of 812 amino acids and has a predicted a molecular mass of 93.5 kDa. It migrates as an approximately 100 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PRKCI protein kinase C, iota [ Homo sapiens ]
• Official Symbol: PRKCI
• Synonyms: PRKCI; protein kinase C, iota; DXS1179E; protein kinase C iota type; PKCI; PRKC-lambda/iota; aPKC-lambda/iota; atypical protein kinase C-lambda/iota; nPKC-iota; MGC26534
• Gene ID: 5584
• mRNA Refseq: NM_002740
• Protein Refseq: NP_002731
• MIM: 600539
• UniProt ID: P41743
• Chromosome Location: 3q26.3
• Pathway: Cell junction organization, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Cell-cell junction organization, organism-specific biosystem; EGFR1 Signaling Pathway, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; G Protein Signaling Pathways, organism-specific biosystem
• Function: ATP binding; metal ion binding; nucleotide binding; phospholipid binding; protein binding; protein kinase C activity; protein kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activity; zinc ion binding