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Recombinant Human PRKCI, GST tagged

Cat.No. : PRKCI-757H
Product Overview : Recombinant Human PRKCI (NP_002731.4) (Met 10-Val 596), fused with the GST tag at the C-terminus, was produced in Baculovirus-Insect cells.
Source : Baculovirus-Insect Cells
Species : Human
Tag : GST
Form : Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM GSH, 0.5mM PMSF, 10% glycerol, pH 7.4
Molecular Mass : The recombinant human PKCλ/GST chimera consists of 812 amino acids and has a predicted a molecular mass of 93.5 kDa. It migrates as an approximately 100 kDa band in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : PRKCI protein kinase C, iota [ Homo sapiens ]
Official Symbol : PRKCI
Synonyms : PRKCI; protein kinase C, iota; DXS1179E; protein kinase C iota type; PKCI; PRKC-lambda/iota; aPKC-lambda/iota; atypical protein kinase C-lambda/iota; nPKC-iota; MGC26534;
Gene ID : 5584
mRNA Refseq : NM_002740
Protein Refseq : NP_002731
MIM : 600539
UniProt ID : P41743
Chromosome Location : 3q26.3
Pathway : Cell junction organization, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Cell-cell junction organization, organism-specific biosystem; EGFR1 Signaling Pathway, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; G Protein Signaling Pathways, organism-specific biosystem;
Function : ATP binding; metal ion binding; nucleotide binding; phospholipid binding; protein binding; protein kinase C activity; protein kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activity; zinc ion binding;

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