||Recombinant human RALA protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.MW = 25.8kDa (227aa).
||RALA (Ras-related protein Ral-A) belongs to the small GTPase superfamily, Ras family of proteins. RALA mediates a distinct downstream signaling pathway from Ras that facilitates cellular transformation. RALA is also thought to be involved in numerous signaling cascades, including regulation of the cytoskeleton, vesicle trafficking, and endocytosis.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol,2mM DTT, 100mM NaCl, 0.1mM PMSF.
||> 90% by SDS - PAGE.
||0.5 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MGSHMAANKP KGQNSLALHK VIMVGSGGVG KSALTLQFMY DEFVEDYEPT KADSYRKKVV LDGEEVQIDI LDTAGQEDYA AIRDNYFRSG EGFLCVFSIT EMESFAATAD FREQILRVKE DENVPFLLVG NKSDLEDKRQ VSVEEAKNRA EQWNVNYVET SAKTRANVDK VFFDLMREIR ARKMEDSKEK NGKKKRKSLA KRIRERC.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Pancreatic cancer; Pathways in cancer; Signalling by NGF