Active Recombinant Human SERPINB2 protein(Met1-Pro415), GST-tagged

• Cat.No.: SERPINB2-3071H
• Product Overview: Recombinant Human SERPINB2 (NP_002566.1) (Met 1-Pro 415) was expressed in Insect Cells, fused with the GST tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: N-GST
• Protein length: Met1-Pro415
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 0.5mM Reduced Glutathione, 10% glycerol, 0.5mM PMSF. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized human GST-SerpinB2 at 10 μg/ml (100 μl/well) can bind biotinylated human uPA-His, The EC50 of biotinylated human uPA-His is 10.24-23.88 ng/ml.
• Molecular Mass: The recombinant human SERPINB2/GST chimera consists of 640 amino acids and predicts a molecular mass of 73 kDa. It migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SERPINB2 serpin peptidase inhibitor, clade B (ovalbumin), member 2 [ Homo sapiens ]
• Official Symbol: SERPINB2
• Synonyms: SERPINB2; serpin peptidase inhibitor, clade B (ovalbumin), member 2; PAI2, PLANH2, serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2; plasminogen activator inhibitor 2; HsT1201; serpin B2; monocyte Arg-serpin; urokinase inhibitor; placental plasminogen activator inhibitor; plasminogen activator inhibitor, type II (arginine-serpin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2; PAI; PAI2; PAI-2; PLANH2
• Gene ID: 5055
• mRNA Refseq: NM_001143818
• Protein Refseq: NP_001137290
• MIM: 173390
• UniProt ID: P05120