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Active Recombinant Human SPARC protein, His-tagged

Cat.No. : SPARC-3193H
Product Overview : Recombinant Human SPARC protein(NP_003109.1)(Met1-Ile303), fused with His tag, was expressed in HEK293.
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Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Bio-activity : Measured by its ability to inhibit the cell growth of Mv-1-Lu mink lung epithelial cells. The ED50 for this effect is typically 1.5-7.5 μg/mL.
Molecular Mass : The secreted recombinant human SPARC consists of 297 amino acids with the predicted molecular mass of 34 kDa. As a result of glycosylation, rhSPARC migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.
Protein Length : Met1-Ile303
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 98 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : SPARC secreted protein, acidic, cysteine-rich (osteonectin) [ Homo sapiens ]
Official Symbol : SPARC
Synonyms : SPARC; secreted protein, acidic, cysteine-rich (osteonectin); ON; cysteine rich protein; osteonectin; BM-40; cysteine-rich protein; basement-membrane protein 40; secreted protein acidic and rich in cysteine;
Gene ID : 6678
mRNA Refseq : NM_003118
Protein Refseq : NP_003109
MIM : 182120
UniProt ID : P09486

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How is the SPARC protein linked to cancer? 04/18/2023

SPARC is associated with cancer progression, as it can influence tumor growth, invasion, and angiogenesis. Its expression levels in tumors are often studied for prognostic and diagnostic purposes.

Can SPARC be a potential therapeutic target for cancer treatment? 12/08/2022

Yes, SPARC is being explored as a therapeutic target for cancer. Researchers are investigating ways to modulate its activity to hinder tumor progression or enhance the efficacy of existing treatments.

In what ways can SPARC expression be detected and measured in clinical settings? 04/25/2021

SPARC expression can be detected through techniques like immunohistochemistry, Western blotting, and molecular assays. These methods help assess SPARC levels in tissues or blood samples.

How does SPARC contribute to tissue repair and regeneration? 03/04/2021

SPARC plays a role in tissue repair by influencing cell-matrix interactions and modulating the extracellular environment. Its involvement in wound healing and tissue regeneration makes it a subject of interest in regenerative medicine.

Are there any ongoing clinical trials targeting SPARC for therapeutic interventions? 03/28/2019

Yes, there are clinical trials exploring the potential of targeting SPARC in various diseases, including cancer. These trials aim to evaluate the safety and efficacy of SPARC-modulating agents.

Customer Reviews (3)

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Reviews
07/18/2021

    The SPARC protein finds great utility in protein electron microscopy structure analysis, consistently delivering exceptional results.

    02/09/2017

      Its remarkable sensitivity and specificity make it a valuable tool for accurate and robust data analysis, bolstering the credibility of research outcomes.

      07/10/2016

        Notably, it exhibits exceptional performance in ELISA assays, providing reliable and precise results in the detection and quantification of target molecules.

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