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Active Recombinant Human THPO, His tagged

Cat.No. : THPO-861H
Product Overview : Recombinant Human THPO (NP_000451.1) precursor (Met 1-Gly 353), fused with a polyhistidine tag at the C-terminus, was produced in CHO Cells.
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Source : CHO
Species : Human
Tag : His
Form : Lyophilized from sterile 50 mM Tris, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 50-200 ng/ml.
Molecular Mass : The recombinant human THPO consists of 343 amino acids with the predicted molecular mass of 36.9 kDa. As a result of glycosylation, the apparent molecular mass of rhTHPO is approximately 60-80 kDa in SDS-PAGE under reducing conditions.
Protein Length : Met 1-Gly 353
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 85 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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