|Product Overview:||Recombinant Ovine Prolactin produced in E.Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids and having a molecular mass of 23 kDa. The Prolactin n is purified by proprietary chromatographic techniques.|
|Description:||Prolactin is a neuroendocrine hormone synthesized primarily by the pituitary gland but also a variety of other cell types including the placenta, brain and uterus. Its primary function is to promote and maintain lactation but has also been shown to have a role in breast cancer development, regulation of reproductive function and immunoregulation.|
|Physical Appearance:||Sterile Filtered White lyophilized (freeze-dried) powder.|
|Purity:||Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.|
|Formulation:||The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.0045 mM NaHCO3.|
|Solubility:||It is recommended to reconstitute the lyophilized Prolactin in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.|
|Amino Acid Sequence:||
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val-Cys-Pro.
Is fully biologically active as evidenced by inducing proliferation of Nb2 cells.
|Protein Content:||Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Prolactin as a Reference Standard.|
|Storage:||Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Prl should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.|
|Gene Name:||PRL prolactin [ Ovis aries ]|