Active Recombinant Mouse Fas protein(Met1-Arg169), His-tagged

• Cat.No.: Fas-3268M
• Product Overview: Recombinant Mouse FAS (NP_032013.2) (Met 1-Arg 169) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Arg169
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit Fas Ligand induced apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0.5-4μg/mL in the presence of 200ng/mL recombinant human Fas ligand.
• Molecular Mass: The recombinant mouse FAS consists of 159 amino acids and has a predicted molecular mass of 18 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFAS is approximately 30-35 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Fas Fas (TNF receptor superfamily member 6) [ Mus musculus ]
• Official Symbol: Fas
• Synonyms: FAS; Fas (TNF receptor superfamily member 6); tumor necrosis factor receptor superfamily member 6; Fas antigen; apo-1 antigen; FASLG receptor; lymphoproliferation; Fas (TNF receptor superfamily member); apoptosis-mediating surface antigen FAS; lpr; APO1; APT1; CD95; TNFR6; Tnfrsf6; AI196731
• Gene ID: 14102
• mRNA Refseq: NM_001146708
• Protein Refseq: NP_001140180