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Recombinant Human Monoglyceride Lipase, His-tagged

Cat. No.: MGLL-2863H
Product Overview: Recombinant human MGLL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. MW = 36.4 kDa(333aa), confirmed by MALDI-TOF.
Description: MGLL is membrane-associated member of the serine hydrolase superfamily. MGLL functions together with hormone-sensitive lipase (LIPE) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides. It is most abundantly expressed in skeletal muscle and adipose tissue.
Source: E.coli.
Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH METGPEDPSS MPEESSPRRT PQSIPYQDLP HLVNADGQYL FCRYWKPTGT PKALIFVSHG AGEHSGRYEE LARMLMGLDL LVFAHDHVGH GQSEGERMVV SDFHVFVRDV LQHVDSMQKD YPGLPVFLLG HSMGGAIAIL TAAERPGHFA GMVLISPLVL ANPESATTFK VLAAKVLNLV LPNLSLGPID SSVLSRNKTE VDIYNSDPLI CRAGLKVCFG IQLLNAVSRV ERALPKLTVP FLLLQGSADR LCDSKGAYLL MELAKSQDKT LKIYEGAYHV LHKELPEVTN SVFHEINMWV SQRTATAGTA SPP
Purity: > 85% by SDS – PAGE.
Form: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol.
Concentration: 0.5 mg/ml (determined by Bradford assay).
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: MGLL monoglyceride lipase [ Homo sapiens ]
Synonyms: MGLL; monoglyceride lipase; MGL; HUK5; MAGL; HU-K5; lysophospholipase-like; monoacylglycerol lipase; lysophospholipase homolog; EC 3.1.1.23
Gene ID: 11343
mRNA Refseq: NM_001003794
Protein Refseq: NP_001003794
MIM: 609699
UniProt ID: Q99685
Chromosome Location: 3q21.3
Pathway: Glycerolipid metabolism; Metabolic pathways
Function: acylglycerol lipase activity; hydrolase activity; lysophospholipase activity; protein homodimerization activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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