||Recombinant human Galectin 2 protein (1-132aa), fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||LGALS2, also known as galectin2, is a soluble beta-galactoside binding lectin that modulates cell-to-cell adhesion and cell-to-extracellular matrix interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. This protein induces apoptosis in activated T cells and binds to the cytokine lymphotoxin-alpha (LTA) with possible implications in risk of myocardial infarction.
||MGSSHHHHHH SSGLVPRGSH MTGELEVKNM DMKPGSTLKI TGSIADGTDG FVINLGQGTD KLNLHFNPRF SESTIVCNSL DGSNWGQEQR EDHLCFSPGS EVKFTVTFES DKFKVKLPDG HELTFPNRLG HSHLSYLSVR GGFNMSSFKL KE.
||Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M Nacl, 1mM DTT, 10% glycerol.
||16.8 kDa (152aa), confirmed by MALDI-TOF.
||> 95% by SDS – PAGE.
||0.5 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.