Recombinant Human WEE1 Protein, GST-HIS6-tagged

• Cat.No.: WEE1-707H
• Product Overview: Recombinant human WEE1, C-terminal fragment, amino acids Q36-Y432, N-terminal GST-HIS6 fusion protein with a Thrombin cleavage site, expressed in Sf9 insect cells. Specific kinase activity (Pi transfer): 2 pmol/µg × min; ATP-KM: 1.6 µM.

Specification

• Description: This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
• Source: Insect cell
• Species: Human
• Tag: GST-HIS6
• Molecular Mass: 74.902 kDa
• Storage: At -80 centigrade. For complete recovery, mix well and spin before use. Product must not be stored in diluted solutions, aliquots below 10 µL are not advisable. Avoid repeated freeze-thaw cycles!
• Concentration: 0.142 µg/µL

Gene Information

• Gene Name: WEE1 WEE1 G2 checkpoint kinase [ Homo sapiens (human) ]
• Official Symbol: WEE1
• Synonyms: WEE1 WEE1 G2 checkpoint kinase; WEE1A; WEE1hu; wee1-like protein kinase; WEE1 homolog; WEE1+ homolog; wee1A kinase; EC 2.7.10.2
• Gene ID: 7465
• mRNA Refseq: NM_001143976
• Protein Refseq: NP_001137448
• MIM: 193525
• UniProt ID: P30291