Recombinant Salmon/Trout Insulin-like Growth Factor I

• Cat.No.: LOC100136741-451S

Specification

• Cat. No.: LOC100136741-451S
• Description: Recombinant salmon (Oncorhynchus kisutch)/ trout (O.mykiss) insulin-like growth factor-I (rsIGF-I) is a 70 amino acid polypeptide. IGF-I stimulates growth and differentiation in most cell types. IGF-I mediates many of the anabolic properties of growth hormone.
• Source: E. coli.
• Molecular Weight: Theoretical: 7704 daltons. By Mass Spectrometry: 7719 daltons. Amino acid sequence and total amino acid analysis confirmed that the recombinant product is salmon/trout IGF-I.
• N-terminal Sequence Analysis Of Recombinant Product: 5 residues >95% single sequence.
• Purity: > 95% (as determined by HPLC and N-terminal sequence analysis).
• Appearance: White Powder.
• Biological Activity: Stimulation of protein synthesis in L6 myoblasts -ED50 32.5 ng/ml.
• Endotoxin (LAL): < 0.1 EU/ug.
• Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20°C. For lower dilutions (e.g.< 1mg/ml) inclusion of a carrier protein is recommended. Avoid freeze-thaw cycles.
• State: Lyophilised from 0.1 M acetic acid: under dry nitrogen @ slight vacuum (-25kPa).
• Stability: At least 2 years at 2-4°C (lyophilised).

Gene Information

• Gene Name: LOC100136741 insulin-like growth factor I [ Oncorhynchus mykiss ]
• Synonyms: insulin-like growth factor I; igf1; LOC100136741; IGF-I; somatomedin
• Gene ID: 100136741
• mRNA Refseq: NM_001124696
• Protein Refseq: NP_001118168
• UniProt ID: Q02815