| Cat.No.: | NUC-0009 |
| Product Name: | Nucleosomes (HeLa Oligo, Biotinylated) |
| Description: | Oligonucleosomes purified from HeLa cells (primarily oligomers of 3-6 units, 600-1200 bp DNA). These are core nucleosomes comprising histone octamers (two copies each of histones H3, H4, H2A, H2B), each wrapped with ~146 bp of DNA with ~50 additional bp of internucleosomal DNA. The purified oligonucleosomes are then biotinylated with an amine-reactive reagent, before dialysis into the final storage buffer. The biotinylation conditions have been optimized to achieve the highest level of modification possible while still retaining most or all of the activity normally observed with a panel of three methyltransferases (Dot1L, NSD2 and MLL1 Complex) when assayed with unmodified nucleosomes. Useful for the assay of various histone methyltransferases by radiolabeled/scintillation proximity techniques with streptavidin-coated reagents or by methods employing a combination of site-specific anti-methyllysine antibodies and streptavidin-conjugated detection reagents. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Source: | HeLa S3 cells |
| Species: | Human |
| Formulation: | Purified biotinylated HeLa oligonucleosomes in 20 mM HEPES pH 7.5, 1 mM EDTA, 0.5 mM PMSF, 1 mM ß-mercaptoethanol, 20% glycerol (w/v). |
| Tag: | Biotin |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Nucleosomes | ||
| NUC-0001 | Recombinant Nucleosome | Inquiry |
| NUC-0002 | Native Nucleosome (HeLa-derived) | Inquiry |
| NUC-0003 | Native Nucleosome (HEK293-derived) | Inquiry |
| NUC-0004 | Recombinant Nucleosome | Inquiry |
| NUC-0005 | Nucleosomes (Chicken Oligo/Poly H5 Enriched) | Inquiry |
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