Recombinant Nucleosome, H3K4ac dNuc


  • Specification
  • Related Products
Cat.No.:  NUC-0032
Product Name:  Recombinant Nucleosome, H3K4ac dNuc
Description:  Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2- Q71DI3 *; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains acetyl-lysine at positions 4. The nucleosome is the basic subunit of chromatin. The 601 sequence is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5’ biotin-TEG group. * H3K4ac also has a Cys to Ala substitution at position 110.
Applications:  Nucleosome, Recombinant Human, H3K4ac dNuc, Biotinylated Nucleosome, Recombinant Human, H3K4ac dNucs are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments.
Storage:  Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles.
Molecular Weight:  199,789 Da.
Species:  Human
Formulation:  Nucleosome, Recombinant Human, H3K4ac (27.3 μg protein weight, 50 μg total weight) in 10 mM Tris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol.
Tag:  Biotin
Expression System:  E. coli
Warning:  This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Online Inquiry

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

USA

Enter your email here to subscribe.

Follow us on

Easy access to products and services you need from our library via powerful searching tools

Copyright © Creative BioMart. All Rights Reserved.