| Cat.No.: | PE-3319 |
| Product Name: | Recombinant Tet3 (824-1795) protein |
| Product Overview: | Recombinant TET3 (824-1795) protein that includes amino acids 824-1795 of human TET3 protein (accession number NP_001274420.1) was expressed in Sf9 cells and contains an N-terminal 6×His-Tag and FLAG-Tag with a molecular weight of 111.3 kDa. The recombinant protein is >30% pure by SDS-PAGE. |
| Background: | TET (Ten Eleven Translocation) family proteins are cytosine oxygenases that catalyze the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). 5hmC can be further oxidized into 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) by TET proteins. Methylation at the C5 position of cytosine is an epigenetic modification of the mammalian genome that plays an important role in transcriptional regulation. TET proteins are responsible for initiating the enzymatic deamination process leading to cytosine demethylation.TET3 plays an important role in DNA methylation reprogramming processes in the mammalian zygote. TET3 may also contribute to somatic cell nuclear reprogramming during animal cloning. Both of CXXC domain and catalytic domain are important for TET3 regulating key genes of Xenopus eye and neural development. |
| Applications: | Suitable for use in cytosine oxygenase assays, enzyme kinetics, inhibitor screening, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP and 0.2 mg/ml 3× Flag peptide. |
| Tag: | His & FLAG |
| Expression System: | Baculovirus |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0154 | Recombinant Human TET3 Cell Lysate | Inquiry |
| ◆ Antibodies | ||
| EAb-0176 | TEAD2 Polyclonal Antibody | Inquiry |
| ◆ Cell Lines | ||
| CL-0215 | Human TET1 Knockout Cell Line 1bp insertion | Inquiry |
| CL-0217 | Human TET2 Knockout Cell Line 19bp deletion | Inquiry |
| CL-0218 | Human TET3 Knockout Cell Line 14bp deletion | Inquiry |
| Related Gene / Proteins | |||
| TEAD1 | TEAD2 | telomerase | TEN1 |
| TENR | TERF2IP | TET | TET1 |
| TET2 | TET3 | ||
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