Recombinant Histone H3K27me3 (MLA)


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Cat.No.:  PE-3557
Product Name:  Recombinant Histone H3K27me3 (MLA)
Product Overview:  Recombinant human Histone H3 trimethyl Lys27 (H3K27me3) (accession number NP_003520.1) was expressed in E. coli cells and contains two substitutions (cysteine to serine at amino acid 96 and cysteine to alanine at amino acid 110). Recombinant methylated histones are specifically methylated via a chemical alkylation reaction that introduces a methyl lysine analog (MLA). The molecular weight of the protein is 15,285 Daltons. The recombinant histone is >92% pure by SDS-PAGE.Protein Sequence (a.a. substitutions in italics): ARTKQTARKS TGGKAPRKQL ATKAARCSAP ATGGVKKPHR YRPGTVALRE IRRYQKSTEL LIRKLPFQRL VREIAQDFKT DLRFQSSAVM ALQEASEAYL VGLFEDTNLA AIHAKRVTIM PKDIQLARRI RGERA
Background:  Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points. Recombinant Histone H3K27me3 (MLA) has been generated using the patented Methylated Lysine Analog (MLA) technology. In MLA, methylated histones are generated via a chemical alkylation reaction that substitutes a methylated analog of lysine, aminoethylcysteine, for the existing lysine at the desired residue. Aminoethylcysteine is structurally and chemically similar to lysine, though it contains a sulfide substitution in place of the lysine Υ-methylene. The MLA technique provides precise control over the site and degree of methylation. The MLA technology is covered under U.S. Patent No. 8,278,112.
Applications:  Suitable for use as positive controls in the analysis of histone post- translational modifiations, as substrates for histone modifiation enzymes, or to generate chromatin in vitro.
Storage:  Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles.
Appearance:  Liquid
Species:  Human
Formulation:  50 mM Tris-HCl, pH 8.0, 150 mM NaCl and 5% glycerol.
Expression System:  E. coli
Warning:  This product is for research use only. Not for use in diagnostic or therapeutic procedures.

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