| Cat.No.: | EKIT-0027 |
| Product Name: | Di-Methyl Histone H3-K4 Quantification Kit (Fluorometric) |
| Product Overview: | Di-Methyl Histone H3-K4 Quantification Kit (Fluorometric) is used for measuring di-methylation of histone H3-K4. |
| Description: | Epigenetic activation or inactivation of genes plays a critical role in many important human diseases, especially in cancer. A major mechanism for epigenetic inactivation of the genes is methylation of CpG islands in genome DNA caused by DNA methyltransferases. Histone methyltransferases (HMTs) control or regulate DNA methylation through chromatin-dependent transcription repression or activation. HMTs transfer 1-3 methyl groups from S-adenosyl-L-methionine to the lysine and arginine residues of histone proteins. SET1, SET7/9, Ash1, ALL-1, MLL, ALR, Trx, and SMYD3 are histone methyltransferases that catalyze methylation of histone H3 at lysine 4 (H3-K4) in mammalian cells. H3-K4 di-methylation is associated with transcriptionally permissive euchromatin regions in the genome and may serve as a epigenetic mark in euchromatin and mediates activated transcription. Increased H3-K4 di-methylation is also found to be involved in some pathological processes such as cancer progression. The H3-K4 di-methylation can be also changed by inhibiton or activation of HMTs. Thus quantitative detection of di-methyl histone H3-K4 would provide usefulinformation for better understanding epigenetic regulation of gene activation and for developing HMT-targeted drugs. The Di-Methyl Histone H3-K4 Quantification Kit (Fluorometric) provides a tool for measuring di-methylation of histone H3-K4. |
| Applications: | For specifically measuring histone H3-K4 di-methylation using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells. |
| Kit Components: | F1 (10X wash buffer) 20 mlF2 (antibody buffer) 12 mlF3 (detection antibody, 1 mg/ml)* 10 µlF4 (fluoro developer)* 24 µlF5 (fluoro enhancer)* 24 µlF6 (fluoro dilutionl)* 8 mlStandard control (100 µg/ml)* 20 µl8 well sample strips (with frame) 98 well standard control strips 3* For maximum recovery of the products, centrifuge the original vial prior to opening the cap. |
| Features & Benefits: | Quick and efficient procedure, which can be finished within 2.5 hours.Innovative fluorometric assay without need for radioactivity, electrophoresis, and chromatography.Specifically capture di-methylated H3-K4 with the detection limit as low as 0.2 ng/well and detection range from 20 ng-5 µg/well of histone extracts.The control is conveniently included for quantification of the amount of di-methylated H3-K4.Strip microplate format makes the assay flexible: manual or high throughput.Simple, reliable, and consistent assay conditions. |
| Storage: | Upon receipt, store F3, F4 and Standard control at –20°C; (2) Store all other components at 4°C away from light. The kit should be stable for 6 months from the shipment date, when stored properly.Note: Check if buffers F1 and F2 contain salt precipitates before using. If so, warm (at room temperature or 37°C) and shake the buffers until the salts are re-dissolved. |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Research Kits | ||
| EKIT-0001 | Acetyl Histone H3-K14 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0002 | Acetyl Histone H3-K14 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0003 | Acetyl Histone H3-K18 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0004 | Acetyl Histone H3-K18 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0005 | Acetyl Histone H3-K23 Quantification Kit (Colorimetric) | Inquiry |
| Related Gene / Proteins | |||
| H3K10 | H3K115 | H3K122 | H3K123 |
| H3K14 | H3K18 | H3K23 | H3K27 |
| H3K36 | H3K37 | H3K4 | H3K5 |
| H3K56 | H3K64 | H3K79 | H3K80 |
| H3K9 | H3R128 | H3R17 | H3R2 More > |
| H3R26 | H3R8 | H3R9 | H3S10 |
| H3S28 | H3S31 | H3S32 | H3T11 |
| H3T118 | H3T3 | H3T32 | H3T41 |
| H3T45 | H3T6 | H3T80 | H3Y41 |
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