| Cat.No.: | EKIT-0081 |
| Product Name: | Methylated DNA Quantification Kit (Colorimetric) |
| Product Overview: | Methylated DNA Quantification Kit (Colorimetric) is used for measuring methylated DNA. |
| Description: | DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer.Methylated DNA Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.It can be paired to use with Hydroxymethylated DNA Quantification Kit (Colorimetric), which specifically quantifies 5-hmC, for simultaneous quantification of both methylated and hydroxymethylated DNA. |
| Applications: | The Methylated DNA Quantification Kit (Colorimetric) is suitable for detecting DNA methylation status using DNA isolated from any species such as mammalians, plants, fungi, bacteria, and viruses in a variety of forms including, but not limited to, cultured cells, fresh and frozen tissues, paraffin-embedded tissues, plasma/serum samples, and body fluid samples. |
| Kit Components: | ME1 (10X Wash Buffer) 14 mlME2 (Binding Solution) 5 mlME3 (Negative Control, 20 μg/ml)* 10 μlME4 ( Positive Control, 20 μg/ml )* 10 μlME5 (Capture Antibody, 1000 μg/ml)* 4 μlME6 (Detection Antibody, 400 μg/ml)* 8 μlME7 (Enhancer Solution) 8 μlME8 (Developer Solution) 5 mlME9 (Stop Solution) 5 mlAssay Strip (with Frame) 48(8x6) wells* Spin the solution down to the bottom prior to use.Note: The ME3 Negative Control is an unmethylated polynucleotide containing 50% of cytosine. The ME4 Positive Control is a methylated polynucleotide containing 50% of 5-methylcytosine. |
| Features & Benefits: | Colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be finished within 4 hours.Innovative kit composition enables background signals to be extremely low, which eliminates the need for plate blocking and allows the assay to be simple, accurate, reliable, and consistent.High sensitivity, of which the detection limit can be as low as 0.2 ng of methylated DNA (methylation).Optimized antibody and enhancer solutions allow high specificity to 5-mC, with no cross-reactivity to unmethylated cytosine and no or negligible cross-reactivity to hydroxymethylcytosine within the indicated concentration range of the sample DNA.Universal positive and negative controls are included, which are suitable for quantifying methylated DNA from any species.Strip-well microplate format makes the assay flexible: manual or high throughput analysis. |
| Detection Method: | Colorimetric |
| Sample Types: | DNA Extract |
| Storage: | Upon receipt: (1) Store ME3, ME4, ME6, and ME7 at –20°C away from light; (2) Store ME1, ME5, ME8, and 8-Well Assay Strips at 4°C away from light; (3) Store remaining components (ME2 and ME9) at room temperature away from light.All components of the kit are stable for 6 months from the date of shipment, when stored properly.Note: Check ME1 (10X Wash Buffer) contains salt precipitates before use. If so, briefly warm at room temperature or 37°C and shake the buffer until the salts are re-dissolved. |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Research Kits | ||
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