EZH2 (Y641N) Chemiluminescent Assay Kit


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Cat.No.:  EKIT-0225
Product Name:  EZH2 (Y641N) Chemiluminescent Assay Kit
Size:  96 reactions
Description:  The EZH2 (Y641N) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (Y641N)/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes. The key to the EZH2 (Y641N) Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Kit Components:  EZH2 (Y641N)/EED/SUZ12/RbAp48/AEBP2: 5 µg; -80°C20 µM S-adenosylmethionine: 250 µl; -80°C Primary Antibody 24: 12.5 µl; -80°C Secondary HRP-labeled antibody 2: 10 µl; -80°C 4x HMT assay buffer 2B: 3 ml; -20°C Blocking buffer: 50 ml; +4°C HRP chemiluminescent substrate A (translucent bottle): 6 ml; +4°C HRP chemiluminescent substrate B (brown bottle): 6 ml; +4°C 96-well plate precoated with histone substrate: 1 plate; +4°C
Warning:  For research use only, not for diagnostic use.
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Related Gene / Proteins
ezh1 EZH2

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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