EZH2 Homogeneous Assay Kit


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Cat.No.:  EKIT-0227
Product Name:  EZH2 Homogeneous Assay Kit
Size:  384 reactions
Product Overview:  EZH2 is a histone methyltransferase that exhibits methylation activity toward H3-K27.
Description:  The EZH2 Homogeneous Assay Kit is designed to measure activity of the EZH2 for screening and profiling applications, using AlphaLISA technology. The key to the EZH2 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing EZH2 enzyme is incubated with the histone substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Kit Components:  EZH2: 100 µg; -80°C100 µM S-adenosylmethionine: 400 µl; -80°C Primary antibody 8*: 5 µl; 4°C Histone mixture (his-tagged): 400 µl; -80°C 4x HMT assay buffer 2: 3 ml; -20°C 4x Detection buffer 2: 2 ml; -20°C
Warning:  For research use only, not for diagnostic use.
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Related Gene / Proteins
ezh1 EZH2

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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