| Cat.No.: | EKIT-0445 |
| Product Name: | Histone H3 (phospho S28) Assay Kit (Colorimetric) |
| Assay Type: | Quantitative |
| Description: | Phosphorylation of histone H3 at serine 28 is conserved through eukaryotes, and an increase in phosphorylation has been shown to correlate with gene activation and cell growth. In vitro studies have shown that phosphorylation of histone H3 at both S10 and S28 is coupled with dynamic acetylation of H3, where histone H3 (phospho S28) had a higher steady state of acetylation than that of H3 (phospho S10). H3 (phospho S28) is regulated by the cell cycle and has been used as a mitotic marker.Histone H3 (phospho S28) Assay Kit (Colorimetric) allows the user to measure global histone H3 (phospho S28) quickly and consistently. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment and it suitable for specifically measuring global histone H3 phophorylation at Ser28 using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells. |
| Background: | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Sensitivity: | 2 ng/well |
| Detection Method: | Colorimetric |
| Target Species: | Mouse, Human; Mammals |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Research Kits | ||
| EKIT-0001 | Acetyl Histone H3-K14 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0002 | Acetyl Histone H3-K14 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0003 | Acetyl Histone H3-K18 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0004 | Acetyl Histone H3-K18 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0005 | Acetyl Histone H3-K23 Quantification Kit (Colorimetric) | Inquiry |
| Related Gene / Proteins | |||
| H3K10 | H3K115 | H3K122 | H3K123 |
| H3K14 | H3K18 | H3K23 | H3K27 |
| H3K36 | H3K37 | H3K4 | H3K5 |
| H3K56 | H3K64 | H3K79 | H3K80 |
| H3K9 | H3R128 | H3R17 | H3R2 More > |
| H3R26 | H3R8 | H3R9 | H3S10 |
| H3S28 | H3S31 | H3S32 | H3T11 |
| H3T118 | H3T3 | H3T32 | H3T41 |
| H3T45 | H3T6 | H3T80 | H3Y41 |
USA
Enter your email here to subscribe.
Follow us on
Easy access to products and services you need from our library via powerful searching tools
