LSD1 (KDM1A) Chemiluminescent Assay Kit


  • Specification
  • Related Products
Cat.No.:  EKIT-0464
Product Name:  LSD1 (KDM1A) Chemiluminescent Assay Kit
Size:  96 reactions
Description:  The LSD1 Chemiluminescent Assay Kit is designed to directly measure activity of human lysine-specific demethylase (LSD1) for screening and profiling applications. The key to the LSD1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing LSD1 enzyme is incubated with a sample containing assay buffer. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Background:  LSD1 is a chromatin-modifying enzyme that specifically removes methyl groups from mono- and dimethylated Lys of histone H3. LSD1 is a critical component of transcriptional regulation via epigenetic histone modifications and is therefore a potential target for drug development.
Applications:  For studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Kit Components:  Kit comes in a convenient format, with a 96-well plate precoated with the methylated histone H3 peptide substrate, primary antibody, the secondary HRP-labeled antibody, demethylase assay buffer, and purified LSD1 for 100 reactions.
Warning:  For research use only, not for diagnostic use.

Online Inquiry

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

USA

Enter your email here to subscribe.

Follow us on

Easy access to products and services you need from our library via powerful searching tools

Copyright © Creative BioMart. All Rights Reserved.