Recombinant Human HDAC3 Cell Lysate


  • Specification
  • Gene Information
  • Related Products
Cat.No.:  EL-0008
Product Name:  Recombinant Human HDAC3 Cell Lysate
Product Overview:  Human HDAC3 / Histone deacetylase 3 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.
Species:  Human
Source:  Baculovirus-Insect Cells
Lysis Buffer:  Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Recommended Usage:  1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage:  Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Warning:  For research use only, not for diagnostic or therapeutic use.
Gene ID NCBI:  8841
Official Symbol:  HDAC3
Official Full Name:  histone deacetylase 3
Synonyms:  HDAC3; histone deacetylase 3; HD3; RPD3; RPD3 2; SMAP45; RPD3-2;
mRNA Refseq:  NM_003883
Protein Refseq:  NP_003874
MIM:  605166
UniProt ID:  O15379
Chromosome Location:  5q31.1-q31.2
Function:  DNA binding; NAD-dependent histone deacetylase activity (H3-K14 specific); NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent histone deacetylase activity (H4-K16 specific); chromatin binding; enzyme binding; histone deacetylase activity; histone deacetylase activity; histone deacetylase activity (H3-K16 specific); histone deacetylase binding; hydrolase activity; protein binding; protein deacetylase activity; transcription corepressor activity; transcription factor binding;

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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