Recombinant Mouse CDK1 Cell Lysate


  • Specification
  • Gene Information
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Cat.No.:  EL-0118
Product Name:  Recombinant Mouse CDK1 Cell Lysate
Product Overview:  Mouse CDC2 Kinase / CDK1 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.
Species:  Mouse
Source:  Baculovirus-Insect Cells
Lysis Buffer:  Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Recommended Usage:  1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage:  Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Warning:  For research use only, not for diagnostic or therapeutic use.
Gene ID NCBI:  12534
Official Symbol:  CDK1
Official Full Name:  cyclin-dependent kinase 1
Synonyms:  CDK1; cyclin-dependent kinase 1; p34 protein kinase; cell division protein kinase 1; cell division cycle 2 homolog A; cell cycle p34 CDC2 kinase protein; cell division cycle control protein 2a; cell division control protein 2 homolog; Cdc2; Cdc2a; p34< cdc2="">
mRNA Refseq:  NM_007659
Protein Refseq:  NP_031685
Function:  ATP binding; Hsp70 protein binding; RNA polymerase II carboxy-terminal domain kinase activity; cyclin binding; cyclin-dependent protein kinase activity; histone kinase activity; kinase activity; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity; transferase activity, transferring phosphorus-containing groups;

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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