K562 Whole Cell Lysate


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Cat.No.:  EL-0246
Product Name:  K562 Whole Cell Lysate
Species:  Human
Source:  K562 (Human Chronic Myelogenous Leukemia) Cells
Preparation Method:  K562 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin). Cell debris was removed by centrifugation.
Recommended Usage:  This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
Storage:  This lysate should be aliquoted in working amounts and may be stored for up to one month at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For longer term storage, freeze working aliquots at -70°C.
Warning:  For research use only, not for diagnostic or therapeutic use.

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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