Human Raji Cytoplasmic Lysate


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Cat.No.:  EL-0263
Product Name:  Human Raji Cytoplasmic Lysate
Species:  Human
Source:  Raji (Human Lymphoma; B Lymphoma) Cells
Preparation Method:  Raji (Human lymphoma; B lymphoma) Cell cytoplasmic protein lysate cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated Raji (Human lymphoma; B lymphoma) cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated Raji (Human lymphoma; B lymphoma) tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.
Recommended Usage:  Raji (Lymphoma; B Lymphoma) Cell Cytoplasmic protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
Storage Buffer:  HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
Storage:  Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Warning:  For research use only, not for diagnostic or therapeutic use.

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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