| Cat.No.: | EL-0329 |
| Product Name: | HeLa acid extract (Paclitaxel treated) |
| Description: | HeLa acid extract (Paclitaxel treated) was prepared under acidic conditions from cells harvested from a cell culture of the HeLa human epithelial carcinoma cell line that was treated with paclitaxel (TAXOL). Acid extraction is performed under conditions that are optimized for solubility of histone proteins. Paclitaxel is one of a class of diterpenoids known as taxoids that have been shown to be effective chemotherapeutic agents in the treatment of solid tumors in humans. The antineoplastic effect is brought on largely by the ability of taxoids to promote stabilization of tubulin polymerization and thus protect microtubules from disassembly. Paclitaxel treatment of HeLa cells is cytotoxic and induces mitotic arrest and apoptotic cell death. |
| Species: | Human |
| Source: | Human Cervix Adenocarcinoma |
| Components: | 2x 50 µg HeLa acid extract (Paclitaxel treated) at 0.5 mg/ml. |
| Extract Composition: | HeLa acid extracts are supplied in dilution buffer (PBS supplemented with 0.5 mM DTT and 2 mM PMSF). Cells were cultured in medium containing 0.1 µg/ml paclitaxel for 20 hours immediately prior to harvesting. |
| Application Notes: | Acid extracts are commonly utilized for isolation and purification of histones. HeLa acid extract (Paclitaxel treated) is specifically recommended for analysis of the mechanisms governing paclitaxel cytotoxicity, particularly in relation to 1) microtubule assembly and motility, 2) mitotic inhibition, 3) cell survival, and 4) cancer therapies. |
| Storage: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0001 | Recombinant Human MBD1 Cell Lysate | Inquiry |
| EL-0002 | Recombinant Human MBD3 Cell Lysate | Inquiry |
| EL-0003 | Recombinant Human MBD3L1 293 Cell Lysate | Inquiry |
| EL-0004 | Recombinant Human MBD4 Lysate | Inquiry |
| EL-0005 | Recombinant Human MBD5 Cell Lysate | Inquiry |
USA
Enter your email here to subscribe.
Follow us on
Easy access to products and services you need from our library via powerful searching tools
