| Cat.No.: | EL-0330 |
| Product Name: | HeLa acid extract (Etoposide treated) |
| Description: | HeLa acid extract (Etoposide treated) was prepared under acidic conditions from cells harvested from a cell culture of the HeLa human epithelial carcinoma cell line that was treated with etoposide. Acid extraction is performed under conditions that are optimized for solubility of histone proteins. Etoposide is a chemotherapeutic agent that acts to inhibit the activity of topoisomerase II, resulting in errors in DNA synthesis that eventually lead to G2 phase arrest and cell death. The topoisomerase II enzyme functions to cleave and unwind the strands of the DNA helix to promote chromosomal disentanglement. Once the DNA strands are unwound, the cut ends are religated. Etoposide forms a ternary complex with DNA and topoisomerase II that prevents religation of the DNA strands, causing DNA damage. The antineoplastic effect of etoposide results from the inefficiency of cancer cells, in comparison to healthy cells, to repair the DNA damage. Thus, the effects of the DNA damage produced by etoposide are compounded and eventually lead to programmed cell death in cancer cells. |
| Species: | Human |
| Source: | Human Cervix Adenocarcinoma |
| Components: | 100 µg HeLa acid extract (Etoposide treated) at 0.5 mg/ml. |
| Extract Composition: | HeLa acid extracts are supplied in dilution buffer (PBS supplemented with 0.5 mM DTT and 2 mM PMSF). Cells were cultured in medium containing 100 µM etoposide for 6 hours immediately prior to harvesting. |
| Application Notes: | Acid extracts are commonly utilized for isolation and purification of histones. |
| Storage: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0001 | Recombinant Human MBD1 Cell Lysate | Inquiry |
| EL-0002 | Recombinant Human MBD3 Cell Lysate | Inquiry |
| EL-0003 | Recombinant Human MBD3L1 293 Cell Lysate | Inquiry |
| EL-0004 | Recombinant Human MBD4 Lysate | Inquiry |
| EL-0005 | Recombinant Human MBD5 Cell Lysate | Inquiry |
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