| Cat.No.: | EL-0332 |
| Product Name: | HeLa acid extract (Anacardic acid treated) |
| Description: | HeLa acid extract (Anacardic acid treated) was prepared under acidic conditions from cells harvested from a cell culture of the HeLa human epithelial carcinoma cell line that cells was treated with anacardic acid. Acid extraction is performed under conditions that are optimized for solubility of histone proteins. Anacardic acid is a compound derived from the shell of a cashew nut that has been shown to have antitumorigenic properties. Recent studies demonstrate that the ability of anacardic acid to act as an inhibitor of histone acetyl transferases (HATs) may attribute to its antineoplastic activity. Although the mechanism by which anacardic acid inhibits HAT activity is unknown, it is suspected that, since anacardic acid shares structural similarities with acetyl-CoA, the HAT acetyl donor, it may function to inhibit binding of acetyl-CoA to the active site of HATs, thus blocking cellular HAT activity. The resulting decrease in acetylation of histones and other cellular proteins alters gene expression and protein function that leads to growth arrest, differentiation, and programmed cell death (apoptosis) of cells. These disruptions in normal cell function also have been shown to cause sensitization of anacardic acid treated cells to ionizing radiation. |
| Species: | Human |
| Source: | Human Cervix Adenocarcinoma |
| Components: | 100 µg HeLa acid extract (Anacardic acid treated) at 0.5 mg/ml. |
| Extract Composition: | HeLa acid extracts are supplied in dilution buffer (PBS supplemented with 0.5 mM DTT and 2 mM PMSF). Cells were cultured in medium containing 30 µM anacardic acid for 6 hours immediately prior to harvesting. |
| Application Notes: | Acid extracts are commonly utilized for isolation and purification of histones. |
| Storage: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
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USA
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