Nb2 nuclear extract


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Cat.No.:  EL-0365
Product Name:  Nb2 nuclear extract
Description:  Nb2 nuclear extract was prepared from cell cultures of the rat lymphoma Nb2 nuclear cell line. The Nb2 cell line was derived from the lymphoma transplant in the thymus of a noble (Nb) strain male rat following prolonged estrogen treatment. Nb2 lymphoma cells are of pre-T cell origin and express the alpha and beta chains of the T cell receptor. However, they lack expression of class II MHC antigens. These cells are dependent on lactogens for mitogenic activity, which can also be stimulated by cytokines and human growth hormone and its analogs. Nb2 cells are most commonly used in studies of lactogen-induced mitogenesis and as a model for tumor progression of T cell cancers.
Species:  Rat
Source:  Rat lymphocytes
Components:  2 x 100 µg of Nb2 nuclear extract at 2.5 µg/µl.
Extract Composition:  Nb2 nuclear extract derived from rat lymphocytes was collected in Lysis Buffer. Lysis Buffer consists of 20 mM Hepes pH 7.9, 100 mM KCl, 20% glycerol, 1 mM MgCl2, 0.5 mM DTT and 0.5 mM PMSF. The protein content has been determined by a Bradford-based assay.
Application Notes:  Nb2 nuclear extract is specifically recommended for studies related to lactogen-induced mitogenesis and T cell cancers.
Storage:  Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Warning:  For research use only, not for diagnostic or therapeutic use.

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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