COS-7 nuclear extract


  • Specification
  • Related Products
Cat.No.:  EL-0407
Product Name:  COS-7 nuclear extract
Description:  COS-7 nuclear extract was prepared from subconfluent undifferentiated cell cultures of the Simian kidney fibroblast COS-7 cell line. The COS-7 cell line was derived from immortalizing the African green monkey CV-1 cell line by transformation with an origin-defective mutant of the SV40 viral genome that is replication-deficient but produces wild-type large T antigen. The large T antigen supports replication and lytic growth of the SV40 virus as well as replication of SV40 promoter-containing plasmids. In addition to supporting propagation of the Simian SV40 virus, the COS-7 cell line is most commonly used in research related to replication and in transfection studies for the production of recombinant proteins.
Species:  Monkey
Source:  Monkey Kidney, SV40 transformed
Components:  2 x 100 µg of COS-7 nuclear extract at 2.5 µg/µl.
Extract Composition:  COS-7 nuclear extract is supplied in lysis buffer (20 mM Hepes (pH 7.5), 400 mM NaCl, 20% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 µM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM beta-glycerophosphate, 1 mM DTT and protease inhibitors).
Application Notes:  COS-7 nuclear extract is specifically recommended for analysis of replication and for transfection studies.
Storage:  Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Warning:  For research use only, not for diagnostic or therapeutic use.

Online Inquiry

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

USA

Enter your email here to subscribe.

Follow us on

Easy access to products and services you need from our library via powerful searching tools

Copyright © Creative BioMart. All Rights Reserved.