3T3-L1 nuclear extract


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Cat.No.:  EL-0416
Product Name:  3T3-L1 nuclear extract
Description:  3T3-L1 nuclear extract was prepared from cell cultures of the mouse embryonic preadipocyte 3T3-L1 cell line. This cell line is derived from the original 3T3-Swiss albino cell line and has a fibroblast-like morphology. However, under appropriate conditions, 3T3-L1 cells are capable of differentiating into an adipocyte-like phenotype and are responsive to lipogenic and lipolytic hormones and drugs. These cells are commonly used as an in vitro model of adipocytes and in studies related to obesity and metabolic diseases.
Species:  Murine
Source:  Murine Embryo Fibroblast
Components:  200 µg of 3T3-L1 nuclear extract at 5.18 µg/µl.
Extract Composition:  Nuclear extracts from 3T3-L1 cells are supplied from embryonic adipose 3T3 cells and are supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).
Application Notes:  3T3-L1 nuclear extract is specifically recommended for studies of 1) adipocyte differentiation, 2) obesity, 3) diabetes, and 4) cardiovascular disease and 5) STATs 1, 3, 5A, 5B and 6.
Storage:  Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Warning:  For research use only, not for diagnostic or therapeutic use.

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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