| Cat.No.: | PE-0276 |
| Product Name: | Recombinant Human DNMT3B, GST-tagged |
| Product Overview: | Recombinant full-length human DNMT3B was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. |
| Description: | DNMT3B or DNA (cytosine-5-)-methyltransferase 3 beta encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. DNMT3B can methylate unmethylated and hemimethylated DNA with equal efficiencies. DNMT3B localizes primarily to the nucleus and its expression is developmentally regulated and is required for genome wide de novo methylation and is essential for mammalian development. Mutations in DNMT3B casue the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. |
| Applications: | Methyltransferase Assay |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | ~138 kDa |
| Purity: | >75% by densitometry |
| Species: | Human |
| Formulation: | Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Tag: | GST |
| Activity: | The specific activity of DNMT3B was determined to be 525 pmol /min/mg |
| Expression System: | Sf9 Insect Cells |
| Concentrations: | 0.1 μg/μl |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | DNMT3B DNA (cytosine-5-)-methyltransferase 3 beta [ Homo sapiens ] |
| Gene ID NCBI: | 1789 |
| Synonyms: | DNMT3B; DNA (cytosine-5-)-methyltransferase 3 beta; DNA (cytosine-5)-methyltransferase 3B; DNA MTase HsaIIIB; DNA methyltransferase HsaIIIB; ICF; ICF1; M.HsaIIIB; |
| mRNA Refseq: | NM_001207055 |
| Protein Refseq: | NP_001193984 |
| MIM: | 602900 |
| UniProt ID: | Q9UBC3 |
| Chromosome Location: | 20q11.2 |
| Function: | DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; DNA binding; DNA-methyltransferase activity; metal ion binding; protein binding; transcription corepressor activity; transcription corepressor activity; transferase activity; unmethylated CpG binding; |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0008 | Vinorelbine Tartrate | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0027 | Recombinant Human DNMT3A 293 Cell Lysate | Inquiry |
| EL-0028 | Recombinant Human DNMT3B 293 Cell Lysate | Inquiry |
| EL-0029 | Recombinant Human DNMT3L 293 Cell Lysate | Inquiry |
| EL-0030 | Recombinant Human DNMT3L 293 Cell Lysate | Inquiry |
| Related Gene / Proteins | |||
| DNA alkyltransferase | DNAJC2 | DNAS1L1 | DNASE1L3 |
| DNMT | DNMT1 | DNMT2 | DNMT3A |
| DNMT3B | DNMT3L | DNMT4 | |
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