| Cat.No.: | NUC-0004 |
| Product Name: | Recombinant Nucleosome |
| Product Overview: | Human recombinant nucleosomes consisting of 147 bp DNA (PCR) and 2 molecules each of histones H2A (a.a. 2-130(end)), H2B (a.a. 2-126(end)), H3 (a.a. 2-137(end)), and H4 (a.a. 2-103(end)), expressed in an E. coli expression system. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. Each histone protein has an N-terminal His-tag. Each H2B has a C-terminal Cys added as a biotinylation site. Total histone MW (before biotinylation) =113.8 kDa. |
| Applications: | Substrate for histone methyltransferase or acetyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases and acetyltransferases for drug discovery and HTS applications. Since these nucleosomes were prepared in E |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 113.8 kDa |
| Species: | Human |
| Formulation: | 20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 1 mM DTT, and 20% glycerol. |
| Tag: | His-tag, Biotin |
| Expression System: | E. coli |
| Format: | Aqueous buffer solution |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene ID NCBI: | NM_033445, NM_003528, NM_003532, NM_003549 |
| UniProt ID: | Q7L7L0, Q16778, P68431, P62805 |
| Product Types | ||
| ◆ Nucleosomes | ||
| NUC-0001 | Recombinant Nucleosome | Inquiry |
| NUC-0002 | Native Nucleosome (HeLa-derived) | Inquiry |
| NUC-0003 | Native Nucleosome (HEK293-derived) | Inquiry |
| NUC-0004 | Recombinant Nucleosome | Inquiry |
| NUC-0005 | Nucleosomes (Chicken Oligo/Poly H5 Enriched) | Inquiry |
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