Active GMP Recombinant Human BMP4 Protein

• Cat.No.: BMP4-135HG
• Product Overview: Recombinant Human BMP4, Ser293-Arg408, was produced in NS0 cell under cGMP guidelines.

Specification

• Source: Mammalian cells
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Bio-activity: Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272.
• Molecular Mass: Predicted Molecular Mass: 13 (monomer) kDa
  SDS-PAGE: 22-25 kDa, reducing conditions; 37-41 kDa, non-reducing conditions
• N-terminal Sequence Analysis: Ser293-Pro-Lys-His-His-Ser-Gln-Arg-Ala-Arg
• Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE with silver staining, under reducing conditions.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 50-200 μg/mL in 4 mM HCl.
• Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Gene Information

• Gene Name: BMP4 bone morphogenetic protein 4 [ Homo sapiens ]
• Official Symbol: BMP4
• Synonyms: BMP4; bone morphogenetic protein 4; BMP2B; BMP-4; BMP-2B; bone morphogenetic protein 2B; ZYME; OFC11; BMP2B1; MCOPS6
• Gene ID: 652
• mRNA Refseq: NM_001202
• Protein Refseq: NP_001193
• MIM: 112262
• UniProt ID: P12644