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Active Recombinant Human IL2 Protein (Ala21-Thr153), GMP Grade, Animal-Free

Cat.No. : IL2-01HG
Product Overview : GMP Recombinant Human IL2 Protein, Ala21-Thr153 (Cys145Ser), with and without an N-terminal Met was expressed in E. coli and produced using non-animal reagents in an animal-free laboratory under cGMP guidelines.
  • Specification
  • Gene Information
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Description : This gene is a member of the interleukin 2 (IL2) cytokine subfamily which includes IL4, IL7, IL9, IL15, IL21, erythropoietin, and thrombopoietin. The protein encoded by this gene is a secreted cytokine produced by activated CD4+ and CD8+ T lymphocytes, that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2R) is a heterotrimeric protein complex whose gamma chain is also shared by IL4 and IL7. The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
Source : E. coli
Species : Human
Bio-activity : Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is 0.0300-0.250 ng/mL.
The specific activity of recombinant human IL-2 is >5.00 × 10^6 IU/mg, which is calibrated against the human IL-2 WHO International Standard.
Molecular Mass : Predicted Molecular Mass: 15.5 kDa
SDS-PAGE: 13 kDa, under reducing conditions.
Protein Length : Ala21-Thr153
N-terminal Sequence Analysis : Ala21 & Met
Endotoxin : < 0.01 EU/μg of the protein by the LAL method.
Purity : > 97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Storage Buffer : Lyophilized from a 0.2 μm filtered solution in Sodium Acetate with Trehalose.
Reconstitution : Reconstitute at 500 μg/mL in sterile deionized water.
Shipping : The product is shipped at ambient temperature.
Gene Name : IL2 interleukin 2 [ Homo sapiens ]
Official Symbol : IL2
Synonyms : IL2; interleukin 2; interleukin-2; IL 2; T cell growth factor; TCGF; aldesleukin; involved in regulation of T-cell clonal expansion; IL-2; lymphokine
Gene ID : 3558
mRNA Refseq : NM_000586
Protein Refseq : NP_000577
MIM : 147680
UniProt ID : P60568

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What is the impact of IL2 on T-cell memory formation? 04/25/2022

IL2 promotes the formation of memory T-cells.

What is the role of IL2 in the tumor microenvironment? 12/12/2021

IL2 has a pro-tumorigenic role in the tumor microenvironment.

How does IL2 interact with other cytokines in a mixed environment? 11/22/2021

IL2 synergizes with IL-12 in a mixed cytokine environment.

Does IL2 modulate the expression of checkpoint inhibitors? 06/03/2020

IL2 upregulates the expression of PD-1 checkpoint inhibitors.

What are the kinetics of IL2 internalization in T cells? 08/18/2019

IL2 is rapidly internalized by activated T-cells.

Does IL2 influence the polarization of macrophages? 07/24/2019

IL2 induces M1 macrophage polarization.

How does IL2 affect the blood-brain barrier permeability? 07/06/2018

IL2 increases blood-brain barrier permeability.

How does IL2's affinity for its receptor change under hypoxic conditions? 04/11/2018

IL2's affinity for its receptor decreases under hypoxic conditions.

What post-translational modifications affect IL2's bioactivity? 02/19/2017

IL2's bioactivity is enhanced by glycosylation.

What is the role of IL2 in the differentiation of Th17 cells? 04/20/2016

IL2 promotes the differentiation of Th17 cells.

Customer Reviews (3)

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Reviews
04/13/2022

    Potent in T-cell activation.

    07/27/2018

      Suitable for cytokine assays.

      01/07/2018

        High stability.

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