|Product Overview:||Recombinant Human NOG, Gln28-Cys232, was produced in NS0 cell under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Bio-activity:||Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.04‑0.2 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP‑4.|
|Molecular Mass:||Predicted Molecular Mass: 23 (monomer) kDa
SDS-PAGE: 30-33 kDa, reducing conditions
|Endotoxin:||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity:||>95%, by SDS-PAGE with silver staining, under reducing conditions.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 100 μg/mL in PBS.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||NOG noggin [ Homo sapiens ]|
|Synonyms:||NOG; noggin; symphalangism 1 (proximal); SYM1; SYNS1;|
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